I00 is an extension characteristic for a file that is one of the parts of a multi-file DVD-Video disc image. Typically, such a file is associated with a tool called DVD Shrink, which is used to back up DVDs containing movies. Sharing such an image makes it possible to transfer such data using smaller storage media.

The most common splitting into files of this type concerns ISO images. The next files get the extension I00, I01, I02, etc.