E01 is a file format used by EnCase Forensic, a software package of tools for forensics analysis. E01 is a disk image file ready for analysis. E01 files are used for securing digital evidence.

EnCase Forensics support many different system files and disk file systems. E01 is an encrypted disk copy stored in binary format. E01 images can be mounted using Vmware virtual machine. EnCase Forensics tool is commonly used by companies, forensics units, military, intelligence agencies, and courts.